Bachelor In Nursing Programs In California more. The way the bioinformatics should be. Program description 454 SFF Workbench is an easy to use SFF file viewer, editor and converter. SFF Workbench is the first and only SFF editor with graphic interface on the entire bioinformatics market! With this tool we wanted to help the biologists to concentrate on their work instead of wasting time poking obscure commands in a console.
Features SFF Viewer: • Display SFF file details (size, total number of samples/reads, key sequence, flow characters) • Display Roche details (index, manifest) • Display the average quality of each sample (before and after end trimming) • Display the sample length (before and after end trimming) • Display quality values • Display low quality/clipped ends in gray color • User-friendly graphic interface. Compact/portable (no installation required, no admin password required • Easy to use file browser (allows you to quickly locate and view SFF files) SFF Editor: • Split huge FastQ/SFF file in chunks of x reads • Convert SFF to FastQ • Convert SFF to Fasta • Convert FastQ to Fasta (multiFasta) • Trim poly-A/T tails • Cut reads with average QV under specified threshold • Cut reads if they contain N bases (the user can specify how many) • Cut reads that are too short • Cut reads that are too long • Cut low complexity reads • Trim low quality ends. Automatically detect and cut low quality bases at the end of each read. • Split multiplexed files (MID/Barcode splitter) • Dereplicate sequences Performance: 454 SFF Workbench loads a 400MB SFF file (containing over 122000 samples) in only 2.5 seconds.
Denoising of 454 Data Sets. Binary sff file. Use the sfffile program from the Roche sfftools package and recreate your fasta and qual files from the truncated. Output from 454 sequencers is generally provided in the form of a Standard Flowgram Format (SFF) file in binary format. 454 Life Sciences (the company that.
It requires only 34MB of memory to display the entire content of the file. The test was performed on a Intel i5 @2.2 GHz, 3GB or RAM, low end hard drive running Windows 7. • It doesn't alter the original SFF file • Buffered files for faster disk access • The SFF index can be saved to external file (when internal index format is unknown) • Automatically append Roche index back to all generated SFF files • Convert SFF to FastQ/multiFASTA • Save as SFF • Sort by 'Average read quality' • Sort by 'Length after clip' • Sort by 'Read name' • Split SFF files in smaller blocks • The user can select and delete multiple reads • Remember last state (GUI position, user settings, last open folder, etc) • Works with very large files (over 6GB). Please help us make SFF Workbench better. Let us know if you have any and we will try to integrate them. The Firm Series more. Your feedback is important to us!
Your email address: Your message: Please enter your feedback here. Anti-spamming counter measure. Please answer: What is 999+1 = Similar bioinformatics tools Name Description DNA Baser is a tool for, DNA sequence analysis, contig editing, and mutation detection.
It also offers a powerful chromatogram viewer/editor. A visual FastQ file viewer, editor and converter. FastQ Workbench is the first and only complete FastQ editor with graphic interface on the entire bioinformatics market! GenBank to FASTA is a freeware program will convert GenBank (gbk) file format to FASTA format.
